pGLO lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 0 no color no color  - pGLO LB/amp 0 no color no color + pGLO LB/amp 1,000 peach color peach color + pGLO LB/amp/ara 100,000 peach color florescent green

2.	What two new traits do your transformed bacteria have?
	they have bigger colony's and one of them can glow
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	I estimate about 100-200 in each one because bacteria are about the size of a cell
4.	What is the role of arabinose in the plates?
	it allows the bacteria to grow under UV lights.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	Fusion tag: observes the activity of the protein
	active indicator: see how proteins change to its environment
	in FERT: to observe the energy transfer
6.	Give an example of another application of genetic engineering.

to see if GFP have any effects an animals.